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Determining the viable cell count is important for calculating dilutions required for the passaging of cells, as well as determining the size and number of flasks needed during growth time. It is also vital when seeding plates for assays, such as the plaque assay , [ 2 ] because the plates need a known number of live replicating cells for the ...
The Miles and Misra Method (or surface viable count) is a technique used in Microbiology to determine the number of colony forming units in a bacterial suspension or homogenate. The technique was first described in 1938 by Miles, Misra and Irwin who at the time were working at the LSHTM. [1] The Miles and Misra method has been shown to be ...
A Coulter counter is an appliance that can count cells as well as measure their volume. It is based on the fact that cells show great electrical resistance; in other words, they conduct almost no electricity. In a Coulter counter the cells, swimming in a solution that conducts electricity, are sucked one by one into a tiny gap.
Theoretically, one viable cell can give rise to a colony through replication. However, solitary cells are the exception in nature, and in most cases the progenitor of a colony is a mass of cells deposited together. [1] [2] In addition, many bacteria grow in chains (e.g. Streptococcus) or clumps (e.g., Staphylococcus). Estimation of microbial ...
If the cells do not have high intracellular potassium and if intracellular sodium is low, then (1) the cell membrane may not be intact, and/or (2) the sodium-potassium pump may not be operating well. [6] [7] Flow cytometry using 7-Aminoactinomycin D (7-AAD), wherein a lower signal indicates viable cells. Therefore, this case shows good ...
When the method only recounts living organisms is called "viable count". [2] There are many methods for the quantification of microorganisms, including microscopy methods, Coulter counter, Mass Spectrometry (for estimating cell mass), and Cell Culture methods which form and grow colonies of bacteria.