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Western blotting can detect hantavirus antigen in tissue samples, [4] but is costly and time consuming. [12] Both traditional and real-time polymerase chain reaction (PCR) tests of blood, saliva, BAL fluids, and tissue samples can be used. There is a possibility of false-negatives with PCR if there are low levels of virus particles in the blood ...
Western blotting can detect hantavirus antigen in tissue samples, [20] but is costly and time consuming. [5] Both traditional and real-time polymerase chain reaction (PCR) tests of blood, saliva, BAL fluids, and tissue samples can be used. There is a possibility of false-negatives with PCR if there are low levels of virus in the blood, and PCR ...
Hantavirus is a family of viruses that can lead to serious illness and death, according to the Centers for Disease Control and Prevention, while Lyssavirus is a group of viruses that can cause rabies.
Differential diagnosis often requires detailed patient history, including recent exposure to rodent habitats, which the patient may not be aware of. Point-of-care and other quick molecular diagnostic tests for hantavirus are under development, which could improve rapid diagnosis in rural areas, typically where most hantavirus cases are found.
The loss of fluid is so severe that the density of blood cells in blood increases due to the loss of liquid (hemoconcentration). Hemoconcentration was observed in several cases and combined with the CDC's findings, a hantavirus was suspected of causing the outbreak. [8] Suspecting a hantavirus required a substantial leap of thought at the time.
Some viruses attach to molecules present on the surface of red blood cells, for example, influenza virus. [10] A consequence of this is that – at certain concentrations – a viral suspension may bind together (agglutinate) the red blood cells thus preventing them from settling out of suspension. [citation needed]