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A cell strain is derived either from a primary culture or a cell line by the selection or cloning of cells having specific properties or characteristics which must be defined. Cell strains are cells that have been adapted to culture but, unlike cell lines, have a finite division potential.
A test should be invariant between relevant subgroups (e.g., demographic groups) within a larger population. [6] For example, for a test to be used in the United Kingdom, the test and its items should have approximately the same meaning for British males and females.
Human isogenic disease models have been likened to 'patients in a test-tube', since they incorporate the latest research into human genetic diseases and do so without the difficulties and limitations involved in using non-human models. [2] Historically, cells obtained from animals, typically mice, have been used to model cancer-related pathways.
This memory is akin to the type of memory used for police line-ups. The particular task described used to be called "item recognition". Scientists study the rates of hits (correct "yes" responses) related to the rates of false alarms (incorrect "yes" responses) with statistical analysis to develop theories on how the recognition judgement is ...
A skinpatch test has been designed and is used in Canada to measure development of rashes, inflammation, swelling or abnormal tissue growth on human volunteers. [25] Unlike corrosives, substances defined as irritants cause only reversible skin damage. Another approach has been the development of test methods that use cultured human cells.
For many viral infections, different host cell strains may have a characteristic response. Currently, there are many concerns within the research community about the validity and purity of cell strains. Contamination has risen within and among laboratories. CPEs can be used to test the purity of a certain cell line.
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The WI-38 cell line stemmed from earlier work by Hayflick growing human cell cultures. [2]In the early 1960s, Hayflick and his colleague Paul Moorhead at the Wistar Institute in Philadelphia, Pennsylvania discovered that when normal human cells were stored in a freezer, the cells remembered the doubling level at which they were stored and, when reconstituted, began to divide from that level to ...