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Contamination on an agar plate. An agar plate is a Petri dish that contains a growth medium solidified with agar, used to culture microorganisms. Sometimes selective compounds are added to influence growth, such as antibiotics. [1] 96 pinner used to perform spot assays with yeast, fungal or bacterial cells
Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample. From here, the plates are rotated to ensure the samples are uniformly mixing with the agar.
The Petri dish was developed by German physician Julius Richard Petri (after whom the name is given) while working as an assistant to Robert Koch at Berlin University.Petri did not invent the culture dish himself; rather, it was a modified version of Koch's invention [9] which used an agar medium that was developed by Walther Hesse. [10]
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
An agar plate or Petri dish is used to provide a growth medium using a mix of agar and other nutrients in which microorganisms, including bacteria and fungi, can be cultured and observed under the microscope. Agar is indigestible for many organisms so that microbial growth does not affect the gel used and it remains stable.
The Petri dish, widely used in microbiology studies to culture microorganisms. Petri dishes are used as research plates for microbiology studies. The dish is partially filled with warm liquid containing agar, and a mixture of specific ingredients that may include nutrients, blood, salts, carbohydrates, dyes, indicators, amino acids and antibiotics.