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Measurements consist of the time-dependent input of power required to maintain equal temperatures between the sample and reference cells. [ 5 ] In an exothermic reaction, the temperature in the sample cell increases upon addition of ligand .
Swiss Mass Abacus is a calculator of peptide and glycopeptide masses. It is purposefully kept as simple as a basic calculator executing arithmetic operations. TOF-DS Proprietary: Software by Markes International used with BenchTOF time-of-flight mass spectrometers. TopFD Open source
The Dumas method has the advantages of being easy to use and fully automatable. It has been developed into a considerably faster method than the Kjeldahl method, and can take a few minutes per measurement, as compared to the hour or more for Kjeldahl. It also does not make use of toxic chemicals or catalysts.
Volume to mass conversions for some common cooking ingredients; ingredient density g/mL [note 5] metric cup 250 mL imperial cup ≈284 mL U.S. customary cup ≈237 mL [note 6] g oz g oz g oz water [note 7] 1 [note 8] 249–250 8.8 283–284 10 236–237 8.3 [note 9] granulated sugar 0.8 [20] 200 7.0 230 8.0 190 6.7 wheat flour 0.5–0.6 [20 ...
Intuitively, the trust associated with a protein measurement depends on the similarity of ratios from different peptides and the signal level of these measurements. A mathematically rigorous approach called BACIQ, that integrates peptide intensities and peptide-measurement agreement into confidence intervals for protein ratios has emerged. [8]
Label-free quantification experiment with 3 samples, 3 LC-MS files and 5 precursor ions/peptides. Intensities at the peak of the chromatographic peaks are used for quantification in this particular case. Peptides are identified via fragmentation mass spectra, and some of the precursor ions will be quantified, but not mapped to any peptide sequence.
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The peptide masses resulting from the digestion can be determined by mass spectrometry using peptide mass fingerprinting. If this information does not allow unequivocal identification of the protein, its peptides can be subject to tandem mass spectrometry for de novo sequencing. Small changes in mass and charge can be detected with 2D-PAGE.