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Most of the dyes commonly used in microscopy are available as BSC-certified stains. This means that samples of the manufacturer's batch have been tested by an independent body, the Biological Stain Commission ( BSC ), and found to meet or exceed certain standards of purity, dye content and performance in staining techniques ensuring more ...
Positive histologic stains that aid in the diagnosis of conditions of or affecting the human integumentary system Stain Cell, material, and/or structure(s) stained Condition(s) in which stain is positive Actin-specific enolase: Infantile digital fibromatosis: AE1/AE3: Squamous cell carcinoma: Alcian blue: Lipoid proteinosis Papular mucinosis ...
The H&E staining procedure is the principal stain in histology [3] [7] [2] [5] in part because it can be done quickly, [7] is not expensive, and stains tissues in such a way that a considerable amount of microscopic anatomy [9] [10] is revealed, [7] [5] [4] and can be used to diagnose a wide range of histopathologic conditions. [8]
The term "immunostaining" was originally used to refer to the immunohistochemical staining of tissue sections, as first described by Albert Coons in 1941. [1] However, immunostaining now encompasses a broad range of techniques used in histology, cell biology, and molecular biology that use antibody-based staining methods.
[1] [10] [11] When paired, this staining procedure is known as H&E staining and is one of the most commonly used combinations in histology. [1] [7] [12] [13] [14] In addition to its use in the H&E stain, haematoxylin is also a component of the Papanicolaou stain (or Pap stain) which is widely used in the study of cytology specimens. [1] [14]
Methods for mordant application depend on the desired stain and tissues under study; pre-, meta- and post-mordanting techniques are used as required. The most commonly used stain used in diagnostic histology of animal tissues is Harris' haematoxylin as part of a haematoxylin and eosin (H&E) stain.
Romanowsky-type stains are widely used in the examination of blood, in the form of blood films, and in the microscopic examination of bone marrow biopsies and aspirate smears. [1] [23] Examination of both blood and bone marrow can be of importance in the diagnosis of a variety of blood diseases. [1] [23] In the United States the Wright and ...
In histology, histopathology, and clinical pathology, Perls Prussian blue is a commonly used method to detect the presence of iron in tissue or cell samples. [1]: 235 [2] [3] [4] Perls Prussian Blue derives its name from the German pathologist Max Perls (1843–1881), who described the technique in 1867. [2]