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3D cell culture. A 3D cell culture is an artificially created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions. Unlike 2D environments (e.g. a Petri dish), a 3D cell culture allows cells in vitro to grow in all directions, similar to how they would in vivo. [1]
There are a variety of platforms used to facilitate the growth of three-dimensional cellular structures including scaffold systems such as hydrogel matrices [69] and solid scaffolds, and scaffold-free systems such as low-adhesion plates, nanoparticle facilitated magnetic levitation, [70] hanging drop plates, [71] [72] and rotary cell culture ...
A Hollow fiber bioreactor is a 3 dimensional cell culturing system based on hollow fibers, which are small, semi-permeable capillary membranes arranged in parallel array with a typical molecular weight cut-off (MWCO) range of 10-30 kDa. These hollow fiber membranes are often bundled and housed within tubular polycarbonate shells to create ...
Cerebral organoid. A neural, or brain organoid, describes an artificially grown, in vitro, tissue resembling parts of the human brain. Neural organoids are created by culturing pluripotent stem cells into a three-dimensional culture that can be maintained for years. [1][2] The brain is an extremely complex system of heterogeneous tissues and ...
The nano-scaffold is a three-dimensional structure composed of polymer fibers very small that are scaled from a Nanometer (10 −9 m) scale. [1] Developed by the American military, the medical technology uses a microscopic apparatus made of fine polymer fibers called a scaffold. [2] Damaged cells grip to the scaffold and begin to rebuild ...
Blood vessels have been observed to grow through the scaffold, thus the scaffold and cells are being integrated into the host tissue. A combination of engineered scaffolds presents an option for a 3D scaffold that can have both the necessary patterns for cell adhesion and the flexibility to adapt to the ever changing environment at the injury site.
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