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  2. Reversed-phase chromatography - Wikipedia

    en.wikipedia.org/wiki/Reversed-phase_chromatography

    A mobile phase in reversed-phase chromatograpy consists of mixtures of water or aqueous buffers, to which organic solvents are added, to elute analytes from a reversed-phase column in a selective manner. [7] [27] The added organic solvents must be miscible with water, and the two most common organic solvents used are acetonitrile and methanol.

  3. High-performance liquid chromatography - Wikipedia

    en.wikipedia.org/wiki/High-performance_liquid...

    Reversed phase HPLC (RP-HPLC) [29] is the most widespread mode of chromatography. It has a non-polar stationary phase and an aqueous, moderately polar mobile phase. In the reversed phase methods, the substances are retained in the system the more hydrophobic they are.

  4. Micellar liquid chromatography - Wikipedia

    en.wikipedia.org/wiki/Micellar_liquid_chromatography

    Reverse phase high-performance liquid chromatography (RP-HPLC) involves a non-polar stationary phase, often a hydrocarbon chain, and a polar mobile or liquid phase. The mobile phase generally consists of an aqueous portion with an organic addition, such as methanol or acetonitrile .

  5. Chromatography - Wikipedia

    en.wikipedia.org/wiki/Chromatography

    The mobile phase consists of the sample being separated/analyzed and the solvent that moves the sample through the column. In the case of HPLC the mobile phase consists of a non-polar solvent(s) such as hexane in normal phase or a polar solvent such as methanol in reverse phase chromatography and the sample being separated. The mobile phase ...

  6. Hydrophilic interaction chromatography - Wikipedia

    en.wikipedia.org/wiki/Hydrophilic_interaction...

    Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) [1] is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography. HILIC uses hydrophilic stationary phases with reversed-phase ...

  7. Monolithic HPLC column - Wikipedia

    en.wikipedia.org/wiki/Monolithic_HPLC_column

    From this revolution, the 1950s also saw the advent of paper chromatography, reversed-phase partition chromatography (RPC), and hydrophobic interaction chromatography (HIC). The first gels for use in LC were created using cross-linked dextrans ( Sephadex ) in an attempt to realize Synge's prediction that a unique single-piece stationary phase ...

  8. These are the 6 most important stock market charts technical ...

    www.aol.com/6-most-important-stock-market...

    "The 10-year UST yield has reversed a long-term secular downtrend off the 1981 highs. The higher high above 3.25 ('18 highs) also validates the multi-decade reversal. Historically, post uptrend or ...

  9. Phenyl isothiocyanate - Wikipedia

    en.wikipedia.org/wiki/Phenyl_isothiocyanate

    Phenyl isothiocyanate (PITC) is a reagent used in reversed phase HPLC. PITC is less sensitive than o-phthaldehyde (OPA) and cannot be fully automated. PITC can be used for analysing secondary amines, unlike OPA. It is also known as Edman's reagent and is used in Edman degradation.

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