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A monohybrid cross is a cross between two organisms with different variations at one genetic locus of interest. [ 1 ] [ 2 ] The character(s) being studied in a monohybrid cross are governed by two or multiple variations for a single location of a gene.
In conducting a monohybrid cross, Mendel initiated the experiment with a pair of pea plants exhibiting contrasting traits, one being tall and the other dwarf. Through cross-pollination, the resulting offspring plants manifested the tall trait. These first-generation hybrids were termed F1, with their offspring referred to as Filial or F1 progeny.
A problem is converted to a series of monohybrid crosses, and the results are combined in a tree. However, a tree produces the same result as a Punnett square in less time and with more clarity. The example below assesses another double-heterozygote cross using RrYy x RrYy.
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In fish such as cichlids, the term F1 cross is used for crosses between two different wild-caught individuals that are assumed to be from different genetic lines. [6] Mules are F1 hybrids between horses (mares) and donkeys (jacks); the opposite sex cross results in hinnies. However, such offspring are almost always sterile.
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Mendel found support for this law in his dihybrid cross experiments. In his monohybrid crosses, an idealized 3:1 ratio between dominant and recessive phenotypes resulted. In dihybrid crosses, however, he found a 9:3:3:1 ratios. This shows that each of the two alleles is inherited independently from the other, with a 3:1 phenotypic ratio for each.