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Steady-state fluorescence spectra of the DNA nucleosides normalized to their maximum intensity. The fluorescence spectra of the DNA monomeric chromophores (nucleobases, nucleosides or nucleotides) in neutral aqueous solution, obtained with excitation around 260 nm, peak in the near ultraviolet (300-400 nm); and a long tail, extending all over the visible domain is present in their emission ...
Contamination by phenol can significantly contribute to overestimation of DNA concentration. Absorption at 230 nm can be caused by contamination by phenolate ion, thiocyanates, and other organic compounds. For a pure RNA sample, the A 230:260:280 should be around 1:2:1, and for a pure DNA sample, the A 230:260:280 should be around 1:1.8:1. [9]
The infrared absorption spectrum of NASA laboratory sulfur dioxide ice is compared with the infrared absorption spectra of ices on Jupiter's moon, Io credit NASA, Bernard Schmitt, and UKIRT. Absorption spectroscopy is useful in chemical analysis [5] because of its specificity and its quantitative nature. The specificity of absorption spectra ...
Nucleic acid NMR is the use of NMR spectroscopy to obtain information about the structure and dynamics of nucleic acid molecules, such as DNA or RNA.As of 2003, nearly half of all known RNA structures had been determined by NMR spectroscopy.
The spectra of basic, acid and intermediate pH solutions are shown. The analytical concentration of the dye is the same in all solutions. In spectroscopy, an isosbestic point is a specific wavelength, wavenumber or frequency at which the total absorbance of a sample does not change during a chemical reaction or a physical change of the sample ...
Spectrochemistry is the application of spectroscopy in several fields of chemistry. It includes analysis of spectra in chemical terms, and use of spectra to derive the structure of chemical compounds, and also to qualitatively and quantitively analyze their presence in the sample.
This difference in the ultraviolet absorption spectra between the oxidized and reduced forms of the coenzymes at higher wavelengths makes it simple to measure the conversion of one to another in enzyme assays – by measuring the amount of UV absorption at 340 nm using a spectrophotometer. [10] NAD + and NADH also differ in their fluorescence.
Different DNA conformations—A-DNA, B-DNA, and Z-DNA—exhibit distinct CD spectral signatures due to variations in base stacking and helical geometry. [ 19 ] [ 20 ] Similarly, RNA structures, including stem-loops, pseudoknots, and G-quadruplexes, produce unique CD spectra that reflect their specific folding patterns and base interactions.