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  2. Agar - Wikipedia

    en.wikipedia.org/wiki/Agar

    Agar is typically sold commercially as a powder that can be mixed with water and prepared similarly to gelatin before use as a growth medium. Nutrients are typically added to meet the nutritional needs of the microbes organism, the formulations of which may be "undefined" where the precise composition is unknown, or "defined" where the exact ...

  3. Buffered charcoal yeast extract agar - Wikipedia

    en.wikipedia.org/wiki/Buffered_charcoal_yeast...

    For preparation of BCYE + antibiotics, add membrane-filtered antibiotics and mix. For BCYE + albumin agar, dissolve the albumin in distilled water and filter sterilize before addition to the medium. Dispense 20 mL per 15 X 100-mm Petri dish. The medium must be mixed frequently during the pouring to keep the charcoal particles suspended.

  4. Growth medium - Wikipedia

    en.wikipedia.org/wiki/Growth_medium

    An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]

  5. Nutrient agar - Wikipedia

    en.wikipedia.org/wiki/Nutrient_agar

    Nutrient agar is a general-purpose solid medium supporting growth of a wide range of non-fastidious organisms. It typically contains (mass/volume): [1] 0.5% peptone – this provides organic nitrogen; 0.3% beef extract/yeast extract – the water-soluble content of these contribute vitamins, carbohydrates, nitrogen, and salts

  6. Agar plate - Wikipedia

    en.wikipedia.org/wiki/Agar_plate

    R2A agar, a nonspecific medium, imitates water, so is used for water analysis. Tryptic (trypticase) soy agar (TSA) is a general-purpose medium produced by enzymatic digestion of soybean meal and casein. It is frequently the base medium of other agar types; for example, blood agar plates are made by enriching TSA plates with blood.

  7. Agarose - Wikipedia

    en.wikipedia.org/wiki/Agarose

    It is a linear polymer made up of the repeating unit of agarobiose, which is a disaccharide made up of D-galactose and 3,6-anhydro-L-galactopyranose. [2] [3] Agarose is one of the two principal components of agar, and is purified from agar by removing agar's other component, agaropectin. [4]

  8. Plate count agar - Wikipedia

    en.wikipedia.org/wiki/Plate_count_agar

    Streak the mixed culture back and forth in the first quadrant (top left) of the agar plate. Do not cut the agar, simply scrape the top. Flame the loop to rid of culture residue. Wait for it to cool for the next quadrant. Streaking again. Proceed to the second quadrant with streaking. Streaks on the medium will overlap.

  9. Isolation (microbiology) - Wikipedia

    en.wikipedia.org/wiki/Isolation_(microbiology)

    To make a subculture, one again works in aseptic technique in microbiology, lifting a single colony off the agar surface with a loop and streaks the material into the 4 quadrants of an agar plate or all over if the colony was singular and did not look mixed. Example of gram staining on a gram positive rod.