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Paper chromatography is a useful technique because it is relatively quick and requires only small quantities of material. Separations in paper chromatography involve the principle of partition. In paper chromatography, substances are distributed between a stationary phase and a mobile phase.
Paper chromatography is a technique that involves placing a small dot or line of sample solution onto a strip of chromatography paper. The paper is placed in a container with a shallow layer of solvent and sealed. As the solvent rises through the paper, it meets the sample mixture, which starts to travel up the paper with the solvent.
A separation process is a method that converts a mixture or a solution of chemical substances into two or more distinct product mixtures, [1] a scientific process of separating two or more substances in order to obtain purity.
Phytochemistry is the study of phytochemicals, which are chemicals derived from plants.Phytochemists strive to describe the structures of the large number of secondary metabolites found in plants, the functions of these compounds in human and plant biology, and the biosynthesis of these compounds.
The history of chromatography spans from the mid-19th century to the 21st.Chromatography, literally "color writing", [1] was used—and named— in the first decade of the 20th century, primarily for the separation of plant pigments such as chlorophyll (which is green) and carotenoids (which are orange and yellow).
Paper chromatography of some spinach leaf extract shows the various pigments present in their chloroplasts: yellowish xanthophylls, greenish chlorophylls a and b. Plants and various other groups of photosynthetic eukaryotes collectively known as "algae" have unique organelles known as chloroplasts.
The leaf is usually the primary site of photosynthesis in plants.. Plants are the eukaryotes that form the kingdom Plantae; they are predominantly photosynthetic.This means that they obtain their energy from sunlight, using chloroplasts derived from endosymbiosis with cyanobacteria to produce sugars from carbon dioxide and water, using the green pigment chlorophyll.
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. [1] This is possible only in certain conditions. It also requires more attention.