Search results
Results From The WOW.Com Content Network
FISH can also be used to compare the genomes of two biological species, to deduce evolutionary relationships. A similar hybridization technique is called a zoo blot. Bacterial FISH probes are often primers for the 16s rRNA region. FISH is widely used in the field of microbial ecology, to identify microorganisms.
Similar to Q-FISH, Flow-FISH is an adaptation of Q-FISH that combines the use of PNAs with flow cytometry. In this method, Flow-FISH uses interphase cells rather than metaphase chromosomes and hybridizes the PNA probes in suspension. Following hybridization, thousands of cells can be analyzed on a flow cytometer in a relatively short time.
The phylogenetic classification of bony fishes is a phylogenetic classification of bony fishes and is based on phylogenies inferred using molecular and genomic data for nearly 2000 fishes.
These tables can be used to examine the genotypical outcome probabilities of the offspring of a single trait (allele), or when crossing multiple traits from the parents. The Punnett square is a visual representation of Mendelian inheritance , a fundamental concept in genetics discovered by Gregor Mendel . [ 10 ]
A metaphase cell positive for the BCR/ABL rearrangement using FISH. Cytogenetics is essentially a branch of genetics, but is also a part of cell biology/cytology (a subdivision of human anatomy), that is concerned with how the chromosomes relate to cell behaviour, particularly to their behaviour during mitosis and meiosis. [1]
Most GM fish are used in basic research in genetics and development. Two species of fish, zebrafish ( Danio rerio ) and medaka (Japanese rice fish, Oryzias latipes ), are most commonly modified because they have optically clear chorions (shells), develop rapidly, the 1-cell embryo is easy to see and micro-inject with transgenic DNA, and ...
Flow-FISH was first published in 1998 by Rufer et al. [11] as a modification of another technique for analyzing telomere length, Q-FISH, that employs peptide nucleic acid probes [12] of a 3'-CCCTAACCCTAACCCTAA-5' sequence labeled with a fluorescin fluorophore to stain telomeric repeats on prepared metaphase spreads of cells that have been treated with colcemid, hypotonic shock, and fixation to ...
Chromogenic in situ hybridization (CISH) is a cytogenetic technique that combines the chromogenic signal detection method of immunohistochemistry (IHC) techniques with in situ hybridization. [ 1 ] [ 2 ] It was developed around the year 2000 as an alternative to fluorescence in situ hybridization (FISH) for detection of HER-2/neu oncogene ...