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[1]: 167–8 Bacteria that produce capsules often have a slimy (mucoid) consistency. [2]: 495 When certain microorganisms are grown on blood agar, they may digest the blood in the medium, causing visible hemolysis (destruction of red blood cells) on the agar plate. In colonial morphology, hemolysis is classified into three types: alpha-, beta ...
An agar plate being viewed in an electronic colony counter Example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England community hospital setting. Different agar plates are used for different specimen ...
The streak plate method [2] helps identify the unknown microbe by producing individual colonies on an agar plate which allows for CFU method to be used: Beginning the streak pattern. Label the base of the plate. Then, visualize the plate in four quadrants: top left (I), top right (II), bottom right (III), bottom left (IV).
Stab cultures are similar to agar plates, but are formed by solid agar in a test tube. Bacteria is introduced via an inoculation needle or a pipette tip being stabbed into the center of the agar. Bacteria grow in the punctured area. [11] Stab cultures are most commonly used for short-term storage or shipment of cultures.
When some otherwise non-hemolytic bacteria, such as strains of the cholera-causing bacteria Vibrio cholerae, are plated on blood agar, no clearings are observed surrounding isolated colonies, but the blood surrounding larger areas of growth turns green. This process, called hemodigestion, is caused by the metabolic by-products of the bacteria ...
This method, which is commonly used with Mueller–Hinton agar, is used by evenly seeding bacteria over a petri dish and applying an antibiotic treated disk to the top of the agar. By observing the ring formed around the disk formed due to the lack of bacterial growth, the zone of inhibition can be found, which is used to find the ...
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