Search results
Results From The WOW.Com Content Network
Vaccinia virus D5 and HSV Primase are examples of AEP-helicase fusion as well. [12] [6] PolpTN2 is an Archaeal primase found in the TN2 plasmid. A fusion of domains homologous to PriS and PriL, it exhibits both primase and DNA polymerase activity, as well as terminal transferase function.
b Shared structural features between archaeal PolD and eukaryotic Polα C-terminal regions. Conserved α-helices and Zn-binding domain are shown in blue and green, respectively. c The cPIP of DP2 resembles the primase-interacting motif located in the C terminus
Shared primase-binding peptide in archaeal PolD and eukaryotic Polα [1] DNA polymerase alpha also known as Pol α is an enzyme complex found in eukaryotes that is involved in initiation of DNA replication. The DNA polymerase alpha complex consists of 4 subunits: POLA1, POLA2, PRIM1, and PRIM2. [2]
Archaeal Genetics is the study of genes that consist of single nucleus-free cells. [24] Archaea have a single, circular chromosomes that contain multiple origins of replication for initiation of DNA synthesis. [25] DNA replication of Archaea involves similar processes including initiation, elongation, and termination.
Archaea feature a simplified version of the ORC, Mcm, and as a consequence the combined pre-RC. Instead of using six different mcm proteins to form a pseudo-symmetrical heterohexamer, all six subunits in the archaeal MCM are the same. They usually have multiple proteins that are homologous to both Cdc6 and Orc1, some of which perform the ...
Structures of archaeal polD and eukaryotic Polα. Not only is the general topology conserved, the two also share a bifunctional primase-and-PCNA-binding PIP-box sequence on the C-terminus, similar to both eukaryotic Polα and Polε. [37] In 1998, the family D of DNA polymerase was discovered in Pyrococcus furiosus and Methanococcus jannaschii. [38]
The Pol α complex (pol α-DNA primase complex) consists of four subunits: the catalytic subunit POLA1, the regulatory subunit POLA2, and the small and the large primase subunits PRIM1 and PRIM2 respectively. Once primase has created the RNA primer, Pol α starts replication elongating the primer with ~20 nucleotides.
The archaeal pre-RC is very different from the bacterial pre-RC and can serve as a simplified model of the eukaryotic pre-RC. It is composed of a single origin recognition complex (ORC) protein, Cdc6 / ORC1 , and a homohexamer of the minichromosome maintenance (MCM) protein.