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A contig (from contiguous) is a set of overlapping DNA segments that together represent a consensus region of DNA. [1] In bottom-up sequencing projects, a contig refers to overlapping sequence data (); [2] in top-down sequencing projects, contig refers to the overlapping clones that form a physical map of the genome that is used to guide sequencing and assembly. [3]
Early de novo sequence assemblers, such as SEQAID [2] (1984) and CAP [3] (1992), used greedy algorithms, such as overlap-layout-consensus (OLC) algorithms. These algorithms find overlap between all reads, use the overlap to determine a layout (or tiling) of the reads, and then produce a consensus sequence.
Only the duplex without overlap at the 5' end will allow extension by DNA polymerase in 3' to 5' direction. Following the extension of the OE-PCR reaction, the PCR mix or the eluted fragments of appropriate size are subject to normal PCR, using the outermost primers used in the initial, mutagenic PCR reactions.
Unidirectional or tandem overlap: the 3' end of one gene overlaps with the 5' end of another gene on the same strand. This arrangement can be symbolized with the notation → → where arrows indicate the reading frame from start to end. Convergent or end-on overlap: the 3' ends of the two genes overlap on opposite strands. This can be written ...
Even though the total number of sub-problems is actually small (only 43 of them), we end up solving the same problems over and over if we adopt a naive recursive solution such as this. Dynamic programming takes account of this fact and solves each sub-problem only once. Figure 2. The subproblem graph for the Fibonacci sequence.
GEO says that about 182,500 people participate in that program daily, but that number could grow significantly. On the company's earnings call, Zoley said GEO believes it has the technology and ...
McDonald’s HACER National Scholarship Program, which was founded in 1985, awards college scholarships to students with at least one Latino parent. The program has awarded more than $33 million ...
Instruction-level parallelism (ILP) is the parallel or simultaneous execution of a sequence of instructions in a computer program. More specifically, ILP refers to the average number of instructions run per step of this parallel execution.