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  2. Trizol - Wikipedia

    en.wikipedia.org/wiki/Trizol

    The correct name of the method is guanidinium thiocyanate-phenol-chloroform extraction. The use of TRIzol can result in DNA yields comparable to other extraction methods, and it leads to >50% bigger RNA yield. [5] [6] An alternative method for RNA extraction is phenol extraction and TCA/acetone precipitation. Chloroform should be exchanged with ...

  3. Acid guanidinium thiocyanate-phenol-chloroform extraction

    en.wikipedia.org/wiki/Acid_guanidinium...

    The RNA is then precipitated in an alcohol (right). Acid guanidinium thiocyanate-phenol-chloroform extraction (abbreviated AGPC) is a liquid–liquid extraction technique in biochemistry and molecular biology. It is widely used for isolating RNA (as well as DNA and protein in some cases).

  4. Eukaryotic translation - Wikipedia

    en.wikipedia.org/wiki/Eukaryotic_translation

    Eukaryotic translation is the biological process by which messenger RNA is translated into proteins in eukaryotes. It consists of four phases: initiation, elongation, termination, and recapping. It consists of four phases: initiation, elongation, termination, and recapping.

  5. RNA extraction - Wikipedia

    en.wikipedia.org/wiki/RNA_extraction

    The extraction of RNA in molecular biology experiments is greatly complicated by the presence of ubiquitous and hardy RNases that degrade RNA samples. Certain RNases can be extremely hardy and inactivating them is difficult compared to neutralizing DNases. In addition to the cellular RNases that are released there are several RNases that are ...

  6. Translation (biology) - Wikipedia

    en.wikipedia.org/wiki/Translation_(biology)

    Overview of eukaryotic messenger RNA (mRNA) translation Translation of mRNA and ribosomal protein synthesis Initiation and elongation stages of translation involving RNA nucleobases, the ribosome, transfer RNA, and amino acids The three phases of translation: (1) in initiation, the small ribosomal subunit binds to the RNA strand and the initiator tRNA–amino acid complex binds to the start ...

  7. Nucleic acid methods - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_methods

    Nucleic acid methods are the techniques used to study ... Minicolumn purification; RNA extraction; Boom method; Synchronous coefficient of drag alteration (SCODA) DNA ...

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