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Ultrasound Localization Microscopy (ULM) is an advanced ultrasound imaging technique. By localizing microbubbles, ULM overcomes the physical limit of diffraction, achieving sub-wavelength level resolution and qualifying as a super-resolution technique. [1] [2] ULM is primarily utilized in vascular imaging.
Functional ultrasound imaging (fUS) is a medical ultrasound imaging technique for detecting or measuring changes in neural activities or metabolism, such as brain activity loci, typically through measuring hemodynamic (blood flow) changes.
Photo-activated localization microscopy (PALM or FPALM) [1] [2] and stochastic optical reconstruction microscopy (STORM) [3] are widefield (as opposed to point scanning techniques such as laser scanning confocal microscopy) fluorescence microscopy imaging methods that allow obtaining images with a resolution beyond the diffraction limit.
English: The main applications and features of functional ultrasound (fUS) imaging. fUS imaging provides (i) a compatibility with a wide range of animal models for preclinical studies, (ii) the ability to image awake and freely moving animals, (iii) possibility to combine with super-resolution ultrasound localization microscopy, (iv) possible extension to 3D imaging, (v) functional ...
Photoacoustic imaging or optoacoustic imaging is a biomedical imaging modality based on the photoacoustic effect.Non-ionizing laser pulses are delivered into biological tissues and part of the energy will be absorbed and converted into heat, leading to transient thermoelastic expansion and thus wideband (i.e. MHz) ultrasonic emission.
Current research in molecular imaging involves cellular/molecular biology, chemistry, and medical physics, and is focused on: 1) developing imaging methods to detect previously undetectable types of molecules, 2) expanding the number and types of contrast agents available, and 3) developing functional contrast agents that provide information ...
In comparison to other super-resolution microscopy techniques such as STORM or PALM that rely on single-molecule localization and hence only allow one active molecule per diffraction-limited area (DLA) and timepoint, [1] [2] SOFI does not necessitate a controlled photoswitching and/ or photoactivation as well as long imaging times.
Multi-spectral. MSOT collects images at multiple wavelengths and resolves the spectral signatures in each voxel imaged, making it a multi-spectral method. Typically, MSOT is used to generate three images: one anatomical image at a single wavelength, one functional image resolving oxy- and deoxy-hemoglobin concentrations, and a third image resolving additional target photoabsorber(s).