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Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]
A stem cell line is a group of stem cells that is cultured in vitro and can be propagated indefinitely. Stem cell lines are derived from either animal or human tissues and come from one of three sources: embryonic stem cells, adult stem cells, or induced pluripotent stem cells. They are commonly used in research and regenerative medicine.
The terminal cell elongates more than the deeper cells; then the production of a lateral bisector takes place in the inner fluid, which tends to divide the cell into two parts, of which the deeper one remains stationary, while the terminal part elongates again, forms a new inner partition, and so on.
To properly segment the Drosophila embryo, the anterior-posterior axis is defined by maternally supplied transcripts giving rise to gradients of these proteins. [ 2 ] [ 3 ] [ 4 ] This gradient then defines the expression pattern for gap genes , which set up the boundaries between the different segments.
The P3 cell divides into P4 and D founder cells. The D founder cells give rise to muscle while the P4 lineage gives rise to the germ line. Axis specification; The anterior/posterior axis is specified by the sperm at the posterior side. At the two cell stage, the anterior cell is the AB cell while the posterior cell is the P1 cell.
All other gene segments between V and D segments are now deleted from the cell's genome. Primary transcript (unspliced RNA) is generated containing the VDJ region of the heavy chain and both the constant mu and delta chains (C μ and C δ). (i.e. the primary transcript contains the segments: V-D-J-C μ-C δ).
When two cells in the Voronoi diagram share a boundary, it is a line segment, ray, or line, consisting of all the points in the plane that are equidistant to their two nearest sites. The vertices of the diagram, where three or more of these boundaries meet, are the points that have three or more equally distant nearest sites.
Recognition of epitopes in a linear fashion. Note: the same (colored) segment of protein can be a part of more than one epitopes. In immunology, a linear epitope (also sequential epitope) is an epitope—a binding site on an antigen—that is recognized by antibodies by its linear sequence of amino acids (i.e. primary structure).