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OpenChrom is an open source software for the analysis and visualization of mass spectrometric and chromatographic data. [4] Its focus is to handle native data files from several mass spectrometry systems (e.g. GC/MS, LC/MS, Py-GC/MS, HPLC-MS), vendors like Agilent Technologies, Varian, Shimadzu, Thermo Fisher, PerkinElmer and others.
The response factor can be expressed on a molar, volume or mass [1] basis. Where the true amount of sample and standard are equal: = where A is the signal (e.g. peak area) and the subscript i indicates the sample and the subscript st indicates the standard. [2]
Chromatography software is called also Chromatography Data System. [1] It is located in the data station of the modern liquid, gas or supercritical fluid chromatographic systems. This is a dedicated software connected to an hardware interface within the chromatographic system, which serves as a central hub for collecting, analyzing, and ...
A calibration curve plot showing limit of detection (LOD), limit of quantification (LOQ), dynamic range, and limit of linearity (LOL).. In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration. [1]
The silver concentration in the test sample is the x-intercept of the plot. The dilution factor is multiplied by this initial concentration to determine the original concentration. Matrix effects occur even with methods such as plasma spectrometry, which have a reputation for being relatively free from interferences. As such, analyst would use ...
The distribution constant (or partition ratio) (K D) is the equilibrium constant for the distribution of an analyte in two immiscible solvents. [1] [2] [3]In chromatography, for a particular solvent, it is equal to the ratio of its molar concentration in the stationary phase to its molar concentration in the mobile phase, also approximating the ratio of the solubility of the solvent in each phase.
A simplified method of calculating chromatogram resolution is to use the plate model. [8] The plate model assumes that the column can be divided into a certain number of sections, or plates and the mass balance can be calculated for each individual plate. This approach approximates a typical chromatogram curve as a Gaussian distribution curve ...
In NMR spectroscopy, e.g. of the nuclei 1 H, 13 C and 29 Si, frequencies depend on the magnetic field, which is not the same across all experiments. Therefore, frequencies are reported as relative differences to tetramethylsilane (TMS), an internal standard that George Tiers proposed in 1958 and that the International Union of Pure and Applied Chemistry has since endorsed.