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A pump is a protein that hydrolyses ATP to transport a particular solute through a membrane, and in doing so, generating an electrochemical gradient membrane potential. This gradient is of interest as an indicator of the state of the cell through parameters such as the Nernst potential .
The pumps deliver the desired flow and composition of the mobile phase through the stationary phase inside the column, then directly into a flow-cell inside the detector. The detector generates a signal proportional to the amount of sample component emerging from the column, hence allowing for quantitative analysis of the sample components.
The basic working principle of an air-operated double diaphragm pump. Cross-section sketch of diaphragm fuel pump Air-operated double diaphragm pump. A diaphragm pump (also known as a Membrane pump) is a positive displacement pump that uses a combination of the reciprocating action of a rubber, thermoplastic or teflon diaphragm and suitable valves on either side of the diaphragm (check valve ...
Different types of pumps are suitable for different applications, for example: a pump's maximum lift height also determines the applications it can be used for. Low-lift pumps are only suitable for the pumping of surface water (e.g., irrigation, drainage of lands, ...), while high-lift pumps allow deep water pumping (e.g., potable water pumping ...
Solute pumping is a form of active transport of a solute through a cell membrane.Solute pumping allows a molecule that cannot regularly cross the lipid bilayer (because of concentration gradient, polarity, or other reasons) to enter the cell by way of a protein channel.
A progressing cavity pump is a type of positive displacement pump and is also known as a progressive cavity pump, progg cavity pump, eccentric screw pump or cavity pump.It transfers fluid by means of the progress, through the pump, of a sequence of small, fixed shape, discrete cavities, as its rotor is turned.
However, because water is a polar molecule this process of simple diffusion is relatively slow, and in tissues with high water permeability the majority of water passes through aquaporin. [ 4 ] [ 5 ] The 2003 Nobel Prize in Chemistry was awarded jointly to Peter Agre for the discovery of aquaporins [ 6 ] and Roderick MacKinnon for his work on ...
Top view of a potassium channel with potassium ions (purple) moving through the pore (in the center). ( PDB : 1BL8 ) Potassium channels have a tetrameric structure in which four identical protein subunits associate to form a fourfold symmetric ( C 4 ) complex arranged around a central ion conducting pore (i.e., a homotetramer).