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ATCC has created and continues to maintain several public databases used for cell line authentication and research. In 2016, ATCC signed an agreement with the National Institutes of Standards and Technology (NIST) to establish standards and a database for short tandem repeat (STR) sequences in mouse and human cell lines. [6]
Authentication should be repeated before freezing cell line stocks, every two months during active culturing and before any publication of research data generated using the cell lines. Many methods are used to identify cell lines, including isoenzyme analysis, human lymphocyte antigen (HLA) typing, chromosomal analysis, karyotyping, morphology ...
[3] [61] The International Cell Line Authentication Committee (ICLAC) notes that many cases of cell line misidentification are the result of cross-contamination of the culture by another, faster-growing cell line. This calls into question the validity of the research done using contaminated cell lines, as certain attributes of the contaminant ...
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The International Cell Line Authentication Committee maintains a list of contaminated cell lines. [10] It includes the cell lines reported above, although usually with reporting dates and articles considerably later than the first dates and articles rising specific concern regarding the respective cell line.
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