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A tank of liquid nitrogen, used to supply a cryogenic freezer (for storing laboratory samples at a temperature of about −150 °C or −238 °F) Controlled-rate and slow freezing, also known as slow programmable freezing (SPF), [18] is a technique where cells are cooled to around -196 °C over the course of several hours.
At least six major areas of cryobiology can be identified: 1) study of cold-adaptation of microorganisms, plants (cold hardiness), and animals, both invertebrates and vertebrates (including hibernation), 2) cryopreservation of cells, tissues, gametes, and embryos of animal and human origin for (medical) purposes of long-term storage by cooling to temperatures below the freezing point of water.
Cryonics (from Greek: κρύος kryos, meaning "cold") is the low-temperature freezing (usually at −196 °C or −320.8 °F or 77.1 K) and storage of human remains in the hope that resurrection may be possible in the future. [1] [2] Cryonics is regarded with skepticism by the mainstream scientific community.
A cryoprotectant is a substance used to protect biological tissue from freezing damage (i.e. that due to ice formation). Arctic and Antarctic insects, fish and amphibians create cryoprotectants (antifreeze compounds and antifreeze proteins) in their bodies to minimize freezing damage during cold winter periods.
Cryoconservation serves as a way to preserve germplasms, which is particularly beneficial for threatened breeds. Indigenous livestock may be conserved for a variety of reasons, including the preservation of local genetics, their importance in local traditions and their value to the culture identity and heritage of the area. [18]
The history of plant cryopreservation started in 1965 when Hirai was studying the biology activities that happened when biological samples were frozen. [1] Three years later, there was the first successful attempt cryopreserving callus cells. [1]
Nitrogen is a liquid under −195.8 °C (77.3 K).. In physics, cryogenics is the production and behaviour of materials at very low temperatures.. The 13th International Institute of Refrigeration's (IIR) International Congress of Refrigeration (held in Washington DC in 1971) endorsed a universal definition of "cryogenics" and "cryogenic" by accepting a threshold of 120 K (−153 °C) to ...
Cryogenic IQF freezers immerse the product in liquid nitrogen at very low temperatures, freezing it rapidly while continuously moving the product to avoid block or lump formation. [5] Although this method shows good freezing results, it might lead to higher processing costs per weight of product due to the cost of the liquid nitrogen required.