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UV-Vis can be used to monitor structural changes in DNA. [8] UV-Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in ...
In optics, the Kubelka–Munk theory devised by Paul Kubelka [1] [2] and Franz Munk, is a fundamental approach to modelling the appearance of paint films. As published in 1931, [3] the theory addresses "the question of how the color of a substrate is changed by the application of a coat of paint of specified composition and thickness, and especially the thickness of paint needed to obscure the ...
Ultraviolet-visible (UV-vis) spectroscopy involves energy levels that excite electronic transitions. Absorption of UV-vis light excites molecules that are in ground-states to their excited-states. [5] Visible region 400–700 nm spectrophotometry is used extensively in colorimetry science. It is a known fact that it operates best at the range ...
With the aid of these rules the UV absorption maximum can be predicted, for example in these two compounds: [8] In the compound on the left, the base value is 214 nm (a heteroannular diene). This diene group has 4 alkyl substituents (labeled 1,2,3,4) and the double bond in one ring is exocyclic to the other (adding 5 nm for an exocyclic double ...
Ultraviolet–visible spectroscopy (UV–vis) can distinguish between enantiomers by showing a distinct Cotton effect for each isomer. UV–vis spectroscopy sees only chromophores , so other molecules must be prepared for analysis by chemical addition of a chromophore such as anthracene .
The goal of absorption spectroscopy techniques (FTIR, ultraviolet-visible ("UV-vis") spectroscopy, etc.) is to measure how much light a sample absorbs at each wavelength. [2] The most straightforward way to do this, the "dispersive spectroscopy" technique, is to shine a monochromatic light beam at a sample, measure how much of the light is ...
A deuterium arc lamp (or simply deuterium lamp) is a low-pressure gas-discharge light source often used in spectroscopy when a continuous spectrum in the ultraviolet region is needed. Plasma "arc" or discharge lamps using hydrogen are notable for their high output in the ultraviolet, with comparatively little output in the visible and infrared.
Figure 1: Simplified schemes of the Variable UV-Vis detector compared to PhotoDiode Array detector. In the Variable UV-Vis the entire optical bench is located before the flow cell whereas in the diode array the flow rate is positioned before the main optical bench. A schematic of the optical systems is shown in Figure 1.