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Tissue clearing is usually useful only combined with one or more fluorescent labeling techniques such as immunolabeling and subsequently imaged, most often by optical sectioning microscopy techniques. [1] [5] [6] Tissue clearing has been applied to many areas in biological research. [7]
3DISCO (stands for “3D imaging of solvent-cleared organs“) [1] is a histological method that make biological samples more transparent (so called “cleared”) by using series of organic solvents for matching the refractive index (RI) of the tissues with that of the surrounding medium.
Clearing technique provides translucent slides via removing part of cytoplasmic content and then applying high refractive index reagents to process the tissues. [2] This method is suitable for preparing whole mount slides. The clearing is a procedure of using clearing reagents for removal of alcohol and makes tissue translucent. [19]
Strongly scattering biological tissue such as brain or kidney has to be chemically fixed and cleared before it can be imaged in a selective plane illumination microscope. [45] Special tissue clearing techniques have been developed for this purpose, e.g. 3DISCO, CUBIC and CLARITY.
In most histology, or histopathology laboratories the dehydration, clearing, and wax infiltration are carried out in tissue processors which automate this process. [13] Once infiltrated in paraffin, tissues are oriented in molds which are filled with wax; once positioned, the wax is cooled, solidifying the block and tissue.
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Tissue embedded within optimal cutting temperature compound (OCT), mounted on a chuck in a cryostat, and ready for section production. The frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen.
Kelly Ripa and Mark Consuelos are mourning the loss of their beloved dog, Chewie, who died on Tuesday, Feb. 4, at the age of 17.. The couple opened up Wednesday's episode of Live with Kelly and ...