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Single-cell omics technologies has extended beyond the transcriptome to profile diverse physical-chemical properties at single-cell resolution, including whole genomes/exomes, DNA methylation, chromatin accessibility, histone modifications, epitranscriptome (e.g., mRNAs, microRNAs, tRNAs, lncRNAs), proteome, phosphoproteome, metabolome, and more.
An extension of this methodology is the integration of single-cell transcriptomes to single-cell methylomes, combining single-cell bisulfite sequencing [12] [13] to single cell RNA-Seq. [14] Other techniques to query the epigenome, as single-cell ATAC-Seq [15] and single-cell Hi-C [16] also exist.
Videomics (or vide-omics): A video analysis paradigm inspired by genomics principles, where a continuous image sequence (or video) can be interpreted as the capture of a single image evolving through time through mutations revealing 'a scene'. Multiomics: Integration of different omics in a single study or analysis pipeline. [28]
Single-cell DNA genome sequencing involves isolating a single cell, amplifying the whole genome or region of interest, constructing sequencing libraries, and then applying next-generation DNA sequencing (for example Illumina, Ion Torrent). Single-cell DNA sequencing has been widely applied in mammalian systems to study normal physiology and ...
In a high-throughput sequencing flow cell, spots are sequenced one nucleotide at a time, with the colour at each round indicating the next nucleotide in the sequence (Illumina Hiseq). Other variations of these techniques use more or fewer colour channels. [51] [101]
A list of more than 100 different single cell sequencing (omics) methods have been published. [1] The large majority of methods are paired with short-read sequencing technologies, although some of them are compatible with long read sequencing.