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An endospore stain of the cell Bacillus subtilis showing endospores as green and the vegetative cell as red Phase-bright endospores of Paenibacillus alvei imaged with phase-contrast microscopy. An endospore is a dormant, tough, and non-reproductive structure produced by some bacteria in the phylum Bacillota.
Endospore staining is a technique used in bacteriology to identify the presence of endospores in a bacterial sample. [1] Within bacteria, endospores are protective structures used to survive extreme conditions, including high temperatures making them highly resistant to chemicals. [ 2 ]
Detects the presence of endospores in six genera of bacteria Endospores: Green Vegetative cells: Red 6 Capsule A: Hiss method (Positive technique) B: Manevals's technique (Negative) Smear stained with Hiss stain following treatment with copper sulphate Bacterial suspension smeared along with Congo red and the Maneval's stain is applied
B. anthracis endospores, in particular, are highly resilient, surviving extremes of temperature, low-nutrient environments, and harsh chemical treatment over decades or centuries. [citation needed] The endospore is a dehydrated cell with thick walls and additional layers that form inside the cell membrane.
The term sporogenesis can also refer to endospore formation in bacteria, which allows the cells to survive unfavorable conditions. Endospores are not reproductive structures and their formation does not require cell fusion or division. Instead, they form through the production of an encapsulating spore coat within the spore-forming cell.
The cells are coccoid. Cells are 1–2.5 μm. Cell division is carried out in two or three successive planes, such that tetrads or packets of eight or more cells are formed. [4] S. ureae forms endospores (like all species of the genus). The endospores are 0.5–1.5 μm. [5] The species can move using a flagellum.
Since the cell wall is required for bacterial survival, but is absent in some eukaryotes, several antibiotics (notably the penicillins and cephalosporins) stop bacterial infections by interfering with cell wall synthesis, while having no effects on human cells which have no cell wall, only a cell membrane.
Two genera of bacterial pathogens are known to produce endospores: the aerobic Bacillus and anaerobic Clostridium. [7] Dipicolinic acid forms a complex with calcium ions within the endospore core. This complex binds free water molecules, causing dehydration of the spore. As a result, the heat resistance of macromolecules within the core increases.