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Downstream processing refers to the recovery and the purification of biosynthetic products, particularly pharmaceuticals, from natural sources such as animal tissue, plant tissue or fermentation broth, including the recycling of salvageable components as well as the proper treatment and disposal of waste.
The reduction of the energy costs of the separation and purification downstream processing and, in particular, to carry out the separation in-situ in the reactor; The use of side products such as hydrogen and carbon dioxide, solid wastes and discharged microorganisms and carry out less expensive process wastewater treatments.
The upstream part of a bioprocess refers to the first step in which microbes/cells are grown, e.g. bacterial or mammalian cell lines (see cell culture), in bioreactors. Upstream processing involves all the steps related to inoculum development, media development, improvement of inoculum by genetic engineering process, optimization of growth ...
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This allows a scientist to grow up large amounts of bacteria or other microorganisms for a variety of downstream applications. Liquid cultures are ideal for preparation of an antimicrobial assay in which the liquid broth is inoculated with bacteria and let to grow overnight (a ‘shaker’ may be used to mechanically mix the broth, to encourage ...
Protein precipitation is widely used in downstream processing of biological products in order to concentrate proteins and purify them from various contaminants. For example, in the biotechnology industry protein precipitation is used to eliminate contaminants commonly contained in blood. [1]
A bioprocess is composed mainly of three stages—upstream processing, bioreaction, and downstream processing—to convert raw material to finished product. [11] The raw material can be of biological or non-biological origin. It is first converted to a more suitable form for processing.
Bioreactor. Biochemical engineering, also known as bioprocess engineering, is a field of study with roots stemming from chemical engineering and biological engineering.It mainly deals with the design, construction, and advancement of unit processes that involve biological organisms (such as fermentation) or organic molecules (often enzymes) and has various applications in areas of interest ...