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Reversed-phase liquid chromatography (RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic compounds. [ 1 ] [ 2 ] [ 3 ] The vast majority of separations and analyses using high-performance liquid chromatography (HPLC) in recent years are done using the ...
A modern self-contained HPLC Schematic representation of an HPLC unit (1) solvent reservoirs, (2) solvent degasser, (3) gradient valve, (4) mixing vessel for delivery of the mobile phase, (5) high-pressure pump, (6) switching valve in "inject position", (6') switching valve in "load position", (7) sample injection loop, (8) pre-column (guard column), (9) analytical column, (10) detector (i.e ...
Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) [1] is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography.
Prior to these studies, HPLC analyses were tuned by modifying the mobile and stationary phases only. Gradient elution for HPLC merely meant changing the ratio of solvents to improve column efficiency, and this requires the use of sophisticated solvent pumping mechanisms along with extra steps and precautions in the chromatographic analysis.
Structural maintenance of chromosomes protein 4 (SMC-4) also known as chromosome-associated polypeptide C (CAP-C) or XCAP-C homolog is a protein that in humans is encoded by the SMC4 gene. [ 5 ] [ 6 ] [ 7 ] SMC-4 is a core subunit of condensin I and II, large protein complexes involved in high order chromosome organization, [ 8 ] including ...
[4] [15] This implies that there must be two epitopes on the protein (one to capture the protein and one to detect the protein) for which specific antibodies are available. [15] Other forward phase protein microarrays directly label the samples, however there is often variability in the labeling efficiency for different protein, and often the ...
Fast protein liquid chromatography (FPLC) is a form of liquid chromatography that is often used to analyze or purify mixtures of proteins. As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a moving fluid (the mobile phase) and a porous solid (the stationary phase).
With the availability of high numbers of genomic sequences it becomes feasible to analyze such sequences for coevolving residues.The effectiveness of this approach results from the fact that a mutation in position i of a protein is more likely to be associated with a mutation in position j than with a back-mutation in i if both positions are functionally coupled (e.g. by taking part in an ...