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The proper time and temperature for dry heat sterilization is 160 °C (320 °F) for 2 hours or 170 °C (340 °F) for 1 hour, and in the case of High Velocity Hot Air sterilisers, 190°C (375°F) for 6 to 12 minutes. [3] Items should be dry before sterilization since water will interfere with the process.
ISO 11138-3:2017 Part 3: Biological indicators for moist heat sterilization processes; ISO 11138-4:2017 Part 4: Biological indicators for dry heat sterilization processes; ISO 11138-5:2017 Part 5: Biological indicators for low-temperature steam and formaldehyde sterilization processes; ISO/TS 11139:2006 Sterilization of health care products ...
Sterilization using steam can also be done at a temperature of 132 C (270 F), at a double pressure. [citation needed] Dry heat sterilization is performed at 170 C (340 F) for one hour or two hours at a temperature of 160 C (320 F). Dry heat sterilization can also be performed at 121 C, for at least 16 hours. [17]
This method is a faster process than dry heat sterilization. Steam sterilization is performed using an autoclave, sometimes called a converter or steam sterilizer. The object or liquid is placed in the autoclave chamber, which is then sealed and heated using pressurized steam to a temperature set point for a defined period of time.
Hot air ovens are electrical devices which use dry heat to sterilize. They were originally developed by Louis Pasteur , [ 1 ] and are essentially the same as fan ovens used for cooking food. Generally, they use a thermostat to control the temperature.
Koch's or Arnold's steam sterilizer: used for steam sterilization A pressure cooker: used as a portable autoclave Biological and chemical indicators: Used to ascertain if a certain process has been completed, e.g. spores used in an autoclave are killed if autoclaving is properly done Filters: •Candle filter: