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The proper time and temperature for dry heat sterilization is 160 °C (320 °F) for 2 hours or 170 °C (340 °F) for 1 hour, and in the case of High Velocity Hot Air sterilisers, 190°C (375°F) for 6 to 12 minutes. [3] Items should be dry before sterilization since water will interfere with the process.
Koch's or Arnold's steam sterilizer: used for steam sterilization A pressure cooker: used as a portable autoclave Biological and chemical indicators: Used to ascertain if a certain process has been completed, e.g. spores used in an autoclave are killed if autoclaving is properly done Filters: •Candle filter:
Sterilization using steam can also be done at a temperature of 132 C (270 F), at a double pressure. [citation needed] Dry heat sterilization is performed at 170 C (340 F) for one hour or two hours at a temperature of 160 C (320 F). Dry heat sterilization can also be performed at 121 C, for at least 16 hours. [17]
ISO 11138-3:2017 Part 3: Biological indicators for moist heat sterilization processes; ISO 11138-4:2017 Part 4: Biological indicators for dry heat sterilization processes; ISO 11138-5:2017 Part 5: Biological indicators for low-temperature steam and formaldehyde sterilization processes; ISO/TS 11139:2006 Sterilization of health care products ...
This method is a faster process than dry heat sterilization. Steam sterilization is performed using an autoclave, sometimes called a converter or steam sterilizer. The object or liquid is placed in the autoclave chamber, which is then sealed and heated using pressurized steam to a temperature set point for a defined period of time.
Hot air ovens are electrical devices which use dry heat to sterilize. They were originally developed by Louis Pasteur , [ 1 ] and are essentially the same as fan ovens used for cooking food. Generally, they use a thermostat to control the temperature.
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Dry heat sterilization which requires a higher temperature and longer time (1–2 hours) than steam autoclave, therefore, only glass or metal objects can be steamed by dry heat. Chemiclave by using Ethylene oxide at relatively low temperature for 2–3 hours.