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Blood components after centrifugation. When blood is collected into a serum-separating tube (SST) and centrifuged, the serum becomes isolated from the red blood cells by a gel acting as a physical barrier to prevent inadvertent remixing of the components.
A tiger top tube after centrifugation to separate blood cells from serum. Vacutainer tubes may contain additional substances that preserve blood for processing in a medical laboratory. Using the wrong tube may make the blood sample unusable for the intended purpose. These additives are typically thin film coatings applied using an ultrasonic ...
After centrifugation, one can distinguish a layer of clear fluid (the plasma), a layer of red fluid containing erythrocytes, and a thin layer in between.Composing less than 1% of the total volume of the blood sample, the buffy coat (so-called because it is usually buff in hue), contains most of the leukocytes and thrombocytes.
A man uses a hand centrifuge to analyse two test tubes with blood, Guinea-Bissau, 1973. Relationships between hematocrit, viscosity, and shear rate are important factors to put into consideration. Since blood is non-Newtonian, the viscosity of the blood is in relation to the hematocrit, and as a function of shear rate.
The tubes have micronized silica particles which help clot the blood before centrifugation, and a gel at the bottom which separates whole blood cells from serum. [1] Silica nanoparticles induce coagulation through contact activation of coagulation factor XII (Hageman factor). [ 2 ]
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Hematocrit measurements had previously been described in the literature, but Wintrobe's method differed in that it used a large tube that could be mass-produced to precise specifications, with a built-in scale. The fraction of red blood cells in the tube was measured after centrifugation to determine the hematocrit. The invention of a ...
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