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The IMViC tests are a group of individual tests used in microbiology lab testing to identify an organism in the coliform group. A coliform is a gram negative , aerobic, or facultative anaerobic rod, which produces gas from lactose within 48 hours.
Simmons' citrate agar (not planted yet) Simmons' citrate agar is used for differentiating gram-negative bacteria on the basis of citrate utilization, [1] especially for distinguishing Gammaproteobacteria of the family Enterobacteriaceae or even between species of the same genus. [2]
Indole test positive: appearance of pink layer at top (e.g. Escherichia coli) Like many biochemical tests on bacteria, results of an indole test are indicated by a change in color following a reaction with an added reagent.
Sulphide Indole Motility (SIM) medium is a bacterial growth medium which tests for the ability to reduce sulfates, the ability to produce indoles, and motility. [1] This combination of challenges in one mixture is convenient and commercially available in stab tubes.
Klebsiella aerogenes, [2] previously known as Enterobacter aerogenes, is a Gram-negative, oxidase-negative, catalase-positive, citrate-positive, indole-negative, rod-shaped bacterium. [3]
Before starting a test, one must confirm the cultured bacteria are Enterobacteriaceae, this is done by a quick oxidase test for cytochrome coxidase. Enterobacteriaceae are typically oxidase negative, meaning they either do not use oxygen as an electron acceptor in the electron transport chain , or they use a different cytochrome enzyme for ...
TSI agar slant results: (from left) preinoculated (as control), P. aeruginosa, E. coli, Salmonella Typhimurium, Shigella flexneri The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test a microorganism's ability to ferment sugars and to produce hydrogen sulfide. [1]
Enterobacteriaceae is a large family of Gram-negative bacteria. It includes over 30 genera and more than 100 species. It includes over 30 genera and more than 100 species. Its classification above the level of family is still a subject of debate, but one classification places it in the order Enterobacterales of the class Gammaproteobacteria in ...