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  2. Sticky and blunt ends - Wikipedia

    en.wikipedia.org/wiki/Sticky_and_blunt_ends

    Blunt ends can also be converted to sticky ends by addition of double-stranded linker sequences containing recognition sequences for restriction endonucleases that create sticky ends and subsequent application of the restriction enzyme or by homopolymer tailing, which refers to extending the molecule's 3' ends with only one nucleotide, allowing ...

  3. List of restriction enzyme cutting sites - Wikipedia

    en.wikipedia.org/wiki/List_of_restriction_enzyme...

    A restriction enzyme or restriction endonuclease is a special type of biological macromolecule that functions as part of the "immune system" in bacteria.One special kind of restriction enzymes is the class of "homing endonucleases", these being present in all three domains of life, although their function seems to be very different from one domain to another.

  4. List of restriction enzyme cutting sites: A - Wikipedia

    en.wikipedia.org/wiki/List_of_restriction_enzyme...

    Name of Restriction Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature, [1] [2] and bibliographical references. Note: When alphabetizing, enzymes are first ordered alphabetically by the acronyms (everything before the roman numeral); then enzymes of a given acronym are ordered alphabetically by the ...

  5. Isocaudomer - Wikipedia

    en.wikipedia.org/wiki/Isocaudomer

    Isocaudomers are pairs of restriction enzymes that have slightly different recognition sequences, but upon cleavage of DNA, generate identical overhanging termini sequences. These sequences can be ligated to one another, but then form an asymmetrical sequence that cannot be cleaved by a restriction enzyme.

  6. Restriction enzyme - Wikipedia

    en.wikipedia.org/wiki/Restriction_enzyme

    A restriction enzyme, restriction endonuclease, REase, ENase or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. [1] [2] [3] Restriction enzymes are one class of the broader endonuclease group of enzymes.

  7. Ligation (molecular biology) - Wikipedia

    en.wikipedia.org/wiki/Ligation_(molecular_biology)

    EcoRI for example generates an AATT end, and since A and T have lower melting temperature than C and G, its melting temperature T m is low at around 6 ° C. [21] For most restriction enzymes, the overhangs generated have a T m that is around 15 ° C. [20] For practical purposes, sticky end ligations are performed at 12-16 ° C, or at room ...

  8. Restriction site - Wikipedia

    en.wikipedia.org/wiki/Restriction_site

    Some restriction enzymes cut DNA at a restriction site in a manner which leaves no overhang, called a blunt end. [2] Blunt ends are much less likely to be ligated by a DNA ligase because the blunt end doesn't have the overhanging base pair that the enzyme can recognize and match with a complementary pair. [3]

  9. BglII - Wikipedia

    en.wikipedia.org/wiki/BglII

    The principal function of restriction enzymes is the protection of the host genome against foreign DNA, but they may also have some involvement in recombination and transposition. [ 1 ] Like most type II restriction enzymes, BglII consists of two identical subunits that form a homodimer around the DNA double helix.