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  2. OLIGO Primer Analysis Software - Wikipedia

    en.wikipedia.org/wiki/OLIGO_Primer_Analysis_Software

    OLIGO Primer Analysis Software is a software for DNA primer design. [1] [2] The first paper describing this software was published in 1989. [3]The program is a real time PCR primer and probe search and analysis tool.

  3. Polymerase chain reaction optimization - Wikipedia

    en.wikipedia.org/wiki/Polymerase_chain_reaction...

    Computer simulations of theoretical PCR results (Electronic PCR) may be performed to assist in primer design. [ 14 ] Touchdown polymerase chain reaction or touchdown style polymerase chain reaction is a method of polymerase chain reaction by which primers will avoid amplifying nonspecific sequences.

  4. In silico PCR - Wikipedia

    en.wikipedia.org/wiki/In_silico_PCR

    The design of appropriate short or long primer pairs is only one goal of PCR product prediction. Other information provided by in silico PCR tools may include determining primer location, orientation, length of each amplicon, simulation of electrophoretic mobility, identification of open reading frames, and links to other web resources. [7] [8] [9]

  5. Multiplex ligation-dependent probe amplification - Wikipedia

    en.wikipedia.org/wiki/Multiplex_ligation...

    And a primer sequence at the end, it is a sequence whose design varies and is what will allow the design of primers and subsequent fragment amplification. In addition, one of the parts of the probe usually contains a stuffer between the target sequence and the primer sequence. The use of different stuffers allows the identification of probes ...

  6. Integrated DNA Technologies - Wikipedia

    en.wikipedia.org/wiki/Integrated_DNA_Technologies

    Joseph A. Walder, M.D., Ph.D. (Northwestern University), founded Integrated DNA Technologies, Inc. in 1987 through a partnership with Baxter Healthcare Corporation at the University of Iowa Technology Innovation Center business incubator. In its first 10 years, IDT grew from a startup with 10 synthesizing machines to a small company with more ...

  7. Multilocus sequence typing - Wikipedia

    en.wikipedia.org/wiki/Multilocus_sequence_typing

    MLST databases contain the reference allele sequences and sequence types for each organism, and also isolate epidemiological data. The websites contain interrogation and analysis software which allow users to query their allele sequences and sequence types. MLST is widely used as a tool for researchers and public healthcare workers.

  8. Amplified fragment length polymorphism - Wikipedia

    en.wikipedia.org/wiki/Amplified_fragment_length...

    Amplified fragment length polymorphism (AFLP-PCR or AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by Pieter Vos, [ 1 ] AFLP uses restriction enzymes to digest genomic DNA , followed by ligation of adaptors to the sticky ends of the restriction ...

  9. List of sequence alignment software - Wikipedia

    en.wikipedia.org/wiki/List_of_sequence_alignment...

    Ultra-fast sequence analysis tool: Both: Edgar, R. C. (2010). "Search and clustering orders of magnitude faster than BLAST". Bioinformatics. 26 (19): 2460– 2461. doi: 10.1093/bioinformatics/btq461. PMID 20709691. publication: 2010 OSWALD OpenCL Smith-Waterman on Altera's FPGA for Large Protein Databases Protein