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  2. Ion chromatography - Wikipedia

    en.wikipedia.org/wiki/Ion_chromatography

    Ion chromatography (or ion-exchange chromatography) is a form of chromatography that separates ions and ionizable polar molecules based on their affinity to the ion exchanger. [1] It works on almost any kind of charged molecule —including small inorganic anions, [ 2 ] large proteins , [ 3 ] small nucleotides , [ 4 ] and amino acids .

  3. Ion exchange - Wikipedia

    en.wikipedia.org/wiki/Ion_exchange

    Industrial and analytical ion-exchange chromatography is another area to be mentioned. Ion-exchange chromatography is a chromatographical method that is widely used for chemical analysis and separation of ions.

  4. Diethylaminoethyl cellulose - Wikipedia

    en.wikipedia.org/wiki/Diethylaminoethyl_cellulose

    Schematic structure of DEAE-C: positively charged diethylaminoethanol groups can bind negative ions. Diethylaminoethyl cellulose (DEAE-C) is a positively charged resin used in ion-exchange chromatography, a type of column chromatography, for the separation and purification of proteins and nucleic acids.

  5. Ion-exchange resin - Wikipedia

    en.wikipedia.org/wiki/Ion-exchange_resin

    Ion-exchange resin beads. An ion-exchange resin or ion-exchange polymer is a resin or polymer that acts as a medium for ion exchange, that is also known as an ionex. [1] It is an insoluble matrix (or support structure) normally in the form of small (0.25–1.43 mm radius) microbeads, usually white or yellowish, fabricated from an organic polymer substrate.

  6. Gibbs–Donnan effect - Wikipedia

    en.wikipedia.org/wiki/Gibbs–Donnan_effect

    Because there is a difference in concentration of ions on either side of the membrane, the pH (defined using the relative activity) may also differ when protons are involved [citation needed]. In many instances, from ultrafiltration of proteins to ion exchange chromatography, the pH of the buffer adjacent to the charged groups of the membrane ...

  7. Chromatography - Wikipedia

    en.wikipedia.org/wiki/Chromatography

    Ion exchange chromatography uses a charged stationary phase to separate charged compounds including anions, cations, amino acids, peptides, and proteins. In conventional methods the stationary phase is an ion-exchange resin that carries charged functional groups that interact with oppositely charged groups of the compound to retain.

  8. Counterion - Wikipedia

    en.wikipedia.org/wiki/Counterion

    Polystyrene sulfonate, a cation-exchange resin, is typically supplied with Na + as the counterion.. In chemistry, a counterion (sometimes written as "counter ion", pronounced as such) is the ion that accompanies an ionic species in order to maintain electric neutrality.

  9. Anion-exchange chromatography - Wikipedia

    en.wikipedia.org/wiki/Anion-exchange_chromatography

    In solution, the resin is coated with positively charged counter-ions . Anion exchange resins will bind to negatively charged molecules, displacing the counter-ion. Anion exchange chromatography is commonly used to purify proteins, amino acids, sugars/carbohydrates and other acidic substances [3] with a negative charge at higher pH levels. The ...

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