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  2. Nucleic acid quantitation - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_quantitation

    The ratio of the absorbance at 260 and 280 nm (A 260/280) is used to assess the purity of nucleic acids. For pure DNA, A 260/280 is widely considered ~1.8 but has been argued to translate - due to numeric errors in the original Warburg paper - into a mix of 60% protein and 40% DNA. [6] The ratio for pure RNA A 260/280 is ~2.0. These ratios are ...

  3. DNA extraction - Wikipedia

    en.wikipedia.org/wiki/DNA_extraction

    The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. [1] DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other contaminants, and ...

  4. Genetic testing - Wikipedia

    en.wikipedia.org/wiki/Genetic_testing

    Genetic testing, also known as DNA testing, is used to identify changes in DNA sequence or chromosome structure. Genetic testing can also include measuring the results of genetic changes, such as RNA analysis as an output of gene expression , or through biochemical analysis to measure specific protein output. [ 1 ]

  5. Qubit fluorometer - Wikipedia

    en.wikipedia.org/wiki/Qubit_fluorometer

    Upon binding to DNA, the dye molecules assume a more rigid shape and increase in fluorescence by several orders of magnitude, most likely due to intercalation between the bases. [ 9 ] [ 10 ] The Qubit fluorometer, a device designed to measure fluorescence signals from samples, operates by correlating these signals with known concentrations of ...

  6. DNA Specimen Provenance Assignment - Wikipedia

    en.wikipedia.org/wiki/DNA_Specimen_Provenance...

    DNA Specimen Provenance Assignment (DSPA) also known as DNA Specimen Provenance Assay, is a molecular diagnostic test used to definitively assign biopsy specimen identity and establish specimen purity during the diagnostic testing cycle for cancer and other histopathological conditions.

  7. Real-time polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Real-time_polymerase_chain...

    Quantitative PCR can also be applied to the detection and quantification of DNA in samples to determine the presence and abundance of a particular DNA sequence in these samples. [3] This measurement is made after each amplification cycle, and this is the reason why this method is called real time PCR (that is, immediate or simultaneous PCR).

  8. Digital polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Digital_polymerase_chain...

    Gene expression and RNA quantification studies have benefited from the increased precision and absolute quantification of dPCR. [91] RNA quantification can be accomplished via RT-PCR , wherein RNA is reverse-transcribed into cDNA in the partitioned reaction itself, and the number of RNA molecules originating from each transcript (or allelic ...

  9. Nucleic acid test - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_test

    Rotavirus. A nucleic acid test (NAT) is a technique used to detect a particular nucleic acid sequence and thus usually to detect and identify a particular species or subspecies of organism, often a virus or bacterium that acts as a pathogen in blood, tissue, urine, etc. NATs differ from other tests in that they detect genetic materials (RNA or DNA) rather than antigens or antibodies.