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Video by Tami (Guy) Port, Chief Executive Nerd at ScienceProfOnline.com. For the lab materials that go with this movie, see the Virtual Microbiology Classroom Microscopy Laboratory Main Page at ScienceProfOnline.com. Date: 26 September 2012: Source: Cheek Epithelial Cells: How to Prepare a Wet Mount Microscope Slide: Author: ScienceProfOnline
A set of standard 75 by 25 mm microscope slides. The white area can be written on to label the slide. A microscope slide (top) and a cover slip (bottom) A microscope slide is a thin flat piece of glass, typically 75 by 26 mm (3 by 1 inches) and about 1 mm thick, used to hold objects for examination under a microscope.
When preparing squashes slides, specimens are supposed to be thin and transparent so that objects can be observed clearly under microscopes. [ 12 ] This technique is to place the material on the glass slide and remove it with the scalpel or to dissect needle, then add a drop of dye solution. [ 2 ]
A single specimen may be a composite of preparations sharing a unique number. An example would be a vertebrate with an alcohol-preserved skin and viscera, a cleared and stained head, the post-cranial dried skeleton, histological, glass slides of various organs, and frozen tissue samples. This specimen could also be a voucher for a publication ...
Without oil, light waves reflect off the slide specimen through the glass cover slip, through the air, and into the microscope lens (see the colored figure to the right). Unless a wave comes out at a 90-degree angle, it bends when it hits a new substance, the amount of bend depending on the angle. This distorts the image.
For example, a common application of cytopathology is the Pap smear, a screening tool used to detect precancerous cervical lesions that may lead to cervical cancer. Cytopathologic tests are sometimes called smear tests because the samples may be smeared across a glass microscope slide [4] for subsequent