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  2. Is your at-home COVID-19 test real? Know how to spot the ...

    www.aol.com/news/home-covid-19-test-real...

    They are less sensitive than a PCR test and are looking for a specific viral antigen that implies there is an infection. Antigen tests can be done in a doctor's office, pharmacy or pop-up testing ...

  3. Polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Polymerase_chain_reaction

    A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.

  4. What Really Causes a False Positive COVID-19 Test? Experts ...

    www.aol.com/lifestyle/false-positive-covid-19...

    In the most basic sense, there are four possible outcomes for a COVID-19 test, whether it’s molecular PCR or rapid antigen: true positive, true negative, false positive, and false negative.

  5. COVID-19 testing - Wikipedia

    en.wikipedia.org/wiki/COVID-19_testing

    Polymerase chain reaction (PCR) is a process that amplifies (replicates) a small, well-defined segment of DNA many hundreds of thousands of times, creating enough of it for analysis. Test samples are treated with certain chemicals [22] [23] that allow DNA to be extracted.

  6. Rapid testing for Omicron: is a nose swab enough? - AOL

    www.aol.com/news/rapid-testing-omicron-nose-swab...

    PCR tests of the saliva from 29 people infected with Omicron detected the virus on average three days before nose samples were positive in antigen, or so-called lateral flow, tests.

  7. Polymerase chain reaction optimization - Wikipedia

    en.wikipedia.org/wiki/Polymerase_chain_reaction...

    The polymerase chain reaction (PCR) is a commonly used molecular biology tool for amplifying DNA, and various techniques for PCR optimization which have been developed by molecular biologists to improve PCR performance and minimize failure.

  8. Helicase-dependent amplification - Wikipedia

    en.wikipedia.org/wiki/Helicase-dependent...

    The polymerase chain reaction is the most widely used method for in vitro DNA amplification for purposes of molecular biology and biomedical research. [1] This process involves the separation of the double-stranded DNA in high heat into single strands (the denaturation step, typically achieved at 95–97 °C), annealing of the primers to the single stranded DNA (the annealing step) and copying ...

  9. Thermostable DNA polymerase - Wikipedia

    en.wikipedia.org/wiki/Thermostable_DNA_Polymerase

    Several DNA polymerases have been described with distinct properties that define their specific utilisation in a PCR, in real-time PCR or in an isothermal amplification. Being DNA polymerases, the thermostable DNA polymerases all have a 5'→3' polymerase activity, and either a 5'→3' or a 3'→5' exonuclease activity.