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These include not only considerations for doing the qPCR itself, but also how the resulting data is collected, analyzed, and presented. An important part of the latter is including information relating to the analysis software used and also submitting the raw data to the relevant databases. [1]
List of protein structure prediction software; Category:Molecular dynamics software; Structural alignment software; Other. Compression of genomic sequencing data; Bioinformatics workflow management system; List of genetic engineering software; List of systems biology visualization software; List of systems biology modelling software; 2D gel ...
Software Description Platform License Developer .NET Bio Language-neutral toolkit built using the Microsoft 4.0 .NET Framework to help developers, researchers, and scientists .NET Framework: Apache: Collaborative project AMPHORA: Metagenomics analysis software Linux: GPL: Jonathan Eisen: Anduril: Component-based workflow framework for data analysis
A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used ...
High resolution melting assays typically involve qPCR amplification followed by a melting curve collected using a fluorescent dye. Due to the sensitivity of high-resolution melting analysis, it is necessary to carefully consider PCR cycling conditions, template DNA quality, and melting curve parameters. [ 12 ]
qPCR is unable to distinguish differences in gene expression or copy number variations that are smaller than twofold. On the other hand, dPCR has a higher precision and has been shown to detect differences of less than 30% in gene expression, distinguish between copy number variations that differ by only 1 copy, and identify alleles that occur ...