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In situ [a] is a Latin phrase meaning "in place" or "on site", derived from in ('in') and situ (ablative of situs, lit. ' place ' ). [ 3 ] The term refers to the examination of phenomena or objects within their original place or context.
It was coined in 1987 as an allusion to the Latin phrases in vivo, in vitro, and in situ, which are commonly used in biology (especially systems biology). The latter phrases refer, respectively, to experiments done in living organisms, outside living organisms, and where they are found in nature.
In situ, Latin phrase meaning on-site or in-place; Situs (law), a legal term meaning "site" where a law applies; In biology, situs refers to the disposition of organs in organisms with left-right asymmetry Situs solitus, the normal position of organs; Situs inversus, the inverted disposition of organs
In situ is a term utilized within a variety of fields meaning "on site" and refers to the location of an event. [2] Within the context of bioremediation, in situ indicates that the location of the bioremediation has occurred at the site of contamination without the translocation of the polluted materials.
Spatial biology is the study of biomolecules and cells in their native three-dimensional context. Spatial biology encompasses different levels of cellular resolution including (1) subcellular localization of DNA, RNA, and proteins, (2) single-cell resolution and in situ communications like cell-cell interactions and cell signaling, (3) cellular neighborhoods, regions, or microenvironments, and ...
In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acid strand (i.e., a probe) to localize a specific DNA or RNA sequence in a portion or section of tissue or if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells ...
In biology, a probe is a single strand of DNA or RNA that is complementary to a nucleotide sequence of interest. RNA probes can be designed for any gene or any sequence within a gene for visualization of mRNA , [ 3 ] [ 4 ] [ 5 ] lncRNA [ 6 ] [ 7 ] [ 8 ] and miRNA in tissues and cells.
In-situ conservation of seed-producing plant species is another conservation strategy. In-situ conservation involves the creation of National Parks, National Forests, and National Wildlife Refuges as a way of preserving the natural habitat of the targeted seed-producing organisms. In-situ conservation of agricultural resources is performed on-farm.