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In situ [a] is a Latin phrase meaning "in place" or "on site", derived from in ('in') and situ (ablative of situs, lit. ' place ' ). [ 3 ] The term refers to the examination of phenomena or objects within their original place or context.
In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acid strand (i.e., a probe) to localize a specific DNA or RNA sequence in a portion or section of tissue or if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells ...
In-situ conservation is the on-site conservation or the conservation of genetic resources in natural populations of plant or animal species, such as forest genetic resources in natural populations of tree species. [1] This process protects the inhabitants and ensures the sustainability of the environment and ecosystem.
In-situ conservation of seed-producing plant species is another conservation strategy. In-situ conservation involves the creation of National Parks, National Forests, and National Wildlife Refuges as a way of preserving the natural habitat of the targeted seed-producing organisms. In-situ conservation of agricultural resources is performed on-farm.
Fluorescence in situ hybridization (FISH) is a laboratory method used to detect and locate a DNA sequence, often on a particular chromosome. [4]In the 1960s, researchers Joseph Gall and Mary Lou Pardue found that molecular hybridization could be used to identify the position of DNA sequences in situ (i.e., in their natural positions within a chromosome).
In biology, a probe is a single strand of DNA or RNA that is complementary to a nucleotide sequence of interest. RNA probes can be designed for any gene or any sequence within a gene for visualization of mRNA , [ 3 ] [ 4 ] [ 5 ] lncRNA [ 6 ] [ 7 ] [ 8 ] and miRNA in tissues and cells.
In situ is a term utilized within a variety of fields meaning "on site" and refers to the location of an event. [2] Within the context of bioremediation, in situ indicates that the location of the bioremediation has occurred at the site of contamination without the translocation of the polluted materials.
Spatial transcriptomics, or spatially resolved transcriptomics, is a method that captures positional context of transcriptional activity within intact tissue. [1] The historical precursor to spatial transcriptomics is in situ hybridization, [2] where the modernized omics terminology refers to the measurement of all the mRNA in a cell rather than select RNA targets.