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The optimum temperature for growth of S. cerevisiae is 30–35 °C (86–95 °F). [16] ... Growth in yeast is synchronized with the growth of the bud, ...
For other organisms used in biological experiments, such as the budding yeast Saccharomyces cerevisiae, a growth temperature of 30 °C (86 °F) is optimal. More elaborate incubators can also include the ability to lower the temperature (via refrigeration), or the ability to control humidity or CO 2 levels.
This strain can grow in NaCl up to 3.0% and some strains up to 6.5% and optimum temperature of 20 and 30 °C. It has also been found that when growth occurs in milk with supplemental yeast extract and glucose, enough acid is produced to curdle milk. Some, but not all strains can ferment citrate.
Most yeasts grow best in a neutral or slightly acidic pH environment. Yeasts vary in regard to the temperature range in which they grow best. For example, Leucosporidium frigidum grows at −2 to 20 °C (28 to 68 °F), Saccharomyces telluris at 5 to 35 °C (41 to 95 °F), and Candida slooffi at 28 to 45 °C (82 to 113 °F). [29]
The optimal temperature for growth is between 25–35 °C (77–95 °F) [4] and growth is enhanced if sugar or fat is added in the medium. Colonies of C. tropicalis are white, smooth and butyrous with a fringed border.
It is particularly tolerant of a wide range of acidity and sugar and salt concentration but is sensitive to temperatures above 35°C unless protected by a small amount of sugar in the medium. [4] In lab, is grown on malt extract agar. After 3 days at 25°C, cells are spheroidal to cylindrical, either singly or in pairs or sometimes in small clumps.
The common incubation conditions are aerobic atmosphere, temperature 25 °C for a period of 5 days. Nevertheless, a higher incubation temperature (30 °C) and shorter incubation time (3 days) can be applied for Z. bailii, as the yeast grows faster at this elevated temperature. [14]
Once the growth medium in the petri dish is inoculated with the desired bacteria, the plates are incubated at the optimal temperature for the growing of the selected bacteria (for example, usually at 37 degrees Celsius, or the human body temperature, for cultures from humans or animals, or lower for environmental cultures). After the desired ...