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Dialysis tubing, also known as Visking tubing, is an artificial semi-permeable membrane tubing [1] used in separation techniques, that facilitates the flow of tiny molecules in solution based on differential diffusion.
Viskase (formerly Visking) is a global corporation based out of Lombard, Illinois, United States that supplies plastic, cellulose, and fibrous film and packaging to the food service industry, including casings for processed meats such as hot dogs and sausages.
Usually, the in-gel digestion is run as an overnight process. For the use of trypsin as protease and a temperature of 37 °C the time of incubation found in most protocols is 12-15 h. However, experiments about the duration of the digestion process showed that after 3 h there is enough material for successful mass spectrometric analysis. [38]
The use of the tube was originally described in 1950, [1] although similar approaches to bleeding varices were described by Westphal in 1930. [2] With the advent of modern endoscopic techniques which can rapidly and definitively control variceal bleeding, Sengstaken–Blakemore tubes are rarely used at present.
Dialysis tubing is the oldest and generally the least expensive format used for dialysis in the lab. Tubing is cut and sealed with a clip at one end, then filled and sealed with a clip on the other end. Tubing provides flexibility but has increased concerns regarding handling, sealing and sample recovery.
It consists, from front to rear, of a hypodermic needle, two bilateral flexible "wings", flexible small-bore transparent tubing (often 20–35 cm long), and lastly a connector (often female Luer). This connector attaches to another device: e.g. syringe , vacuum tube holder/hub, or extension tubing from an infusion pump or gravity-fed infusion ...