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Rucci E, García C, Botella G, De Giusti A, Naiouf M, Prieto-Matías M [11] 2016 parasail Fast Smith-Waterman search using SIMD parallelization: Both: Daily J: 2015 PSI-BLAST: Position-specific iterative BLAST, local search with position-specific scoring matrices, much more sensitive than BLAST: Protein
Rolling circle replication produces multiple copies of a single circular template. Rolling circle replication (RCR) is a process of unidirectional nucleic acid replication that can rapidly synthesize multiple copies of circular molecules of DNA or RNA, such as plasmids, the genomes of bacteriophages, and the circular RNA genome of viroids.
Plasmid miniprep. 0.8% agarose gel ethidium bromide-stained.. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA.It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology.
This page is a subsection of the list of sequence alignment software.. Multiple alignment visualization tools typically serve four purposes: Aid general understanding of large-scale DNA or protein alignments
They are the standard cloning vectors and the ones most commonly used. Most general plasmids may be used to clone DNA inserts of up to 15 kb in size. One of the earliest commonly used cloning vectors is the pBR322 plasmid. Other cloning vectors include the pUC series of plasmids, and a large number of different cloning plasmid vectors are ...
An agarose gel cast in tray, to be used for gel electrophoresis. Agarose gel is a three-dimensional matrix formed of helical agarose molecules in supercoiled bundles that are aggregated into three-dimensional structures with channels and pores through which biomolecules can pass. [3]
Alkaline lysis is the process of isolating plasmid deoxyribonucleic acid (DNA) in bacteria. It is a standard method used in molecular biology to isolate the plasmid without obtaining chromosomal DNA. The first alkaline lysis was performed by Birnom and Doly in 1979. [ 1 ]
Genomic deoxyribonucleic acid (abbreviated as gDNA [1]) is chromosomal DNA, in contrast to extra-chromosomal DNAs like plasmids.Most organisms have the same genomic DNA in every cell; however, only certain genes are active in each cell to allow for cell function and differentiation within the body.