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Minipreparation of plasmid DNA is a rapid, small-scale isolation of plasmid DNA from bacteria. [20] [21] Commonly used miniprep methods include alkaline lysis and spin-column based kits. [3] [22] It is based on the alkaline lysis method. The extracted plasmid DNA resulting from performing a miniprep is itself often called a "miniprep".
Plasmid Cloning Guides Molecular Cloning Guides —References to help scientists design plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning. Molecular Cloning Protocols —Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification ...
[3] Lipopolysaccharides can have substantial impacts on human health, primarily through interactions with the immune system. LPS is a potent activator of the immune system and is a pyrogen (agent that causes fever). [4] In severe cases, LPS can trigger a brisk host response and multiple types of acute organ failure [5] which can lead to septic ...
Inhibitors may be present in the original sample, such as blood, fabrics, tissues and soil but may also be added as a result of the sample processing and DNA extraction techniques used. [3] Excess salts including KCl and NaCl, ionic detergents such as sodium deocycholate , sarkosyl and SDS , ethanol, isopropanol and phenol among others, all ...
Alkaline lysis is the process of isolating plasmid deoxyribonucleic acid (DNA) in bacteria. It is a standard method used in molecular biology to isolate the plasmid without obtaining chromosomal DNA. The first alkaline lysis was performed by Birnom and Doly in 1979. [ 1 ]
The activated region of the delta toxin is composed of three distinct structural domains: an N-terminal helical bundle domain (InterPro: IPR005639) involved in membrane insertion and pore formation; a beta-sheet central domain involved in receptor binding; and a C-terminal beta-sandwich domain (InterPro: IPR005638) that interacts with the N-terminal domain to form a channel.