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APEX2 is a derivative of APEX engineered via yeast surface display directed evolution. APEX2 shows improved labeling efficiency and cellular expression levels. [8] To label proteins nearby a protein of interest, a typical proximity labeling experiment begins by cellular expression of an APEX2 fusion to the protein of interest, which localizes ...
Ascorbate peroxidase (or L-ascorbate peroxidase, APX or APEX) (EC 1.11.1.11) is an enzyme that catalyzes the chemical reaction. L-ascorbate + H 2 O 2 dehydroascorbate + 2 H 2 O. It is a member of the family of heme-containing peroxidases.
Proximity labeling (PL) is a method for discovery of molecules that reside within a few nanometers (1-5 nm) of a designated molecule of interest, within living cells or organisms. The technique involves fusing a promiscuous labeling enzyme to the molecule of interest and then adding a small-molecule substrate that enables the enzyme to ...
Site-directed spin labeling (SDSL) is a technique for investigating the structure and local dynamics of proteins using electron spin resonance. The theory of SDSL is based on the specific reaction of spin labels with amino acids. A spin label's built-in protein structure can be detected by EPR spectroscopy.
A spin label (SL) is an organic molecule which possesses an unpaired electron, usually on a nitrogen atom, and the ability to bind to another molecule. Spin labels are normally used as tools for probing proteins or biological membrane -local dynamics using electron paramagnetic resonance spectroscopy .
In electron diffraction, a diffraction pattern is produced by the interaction of the electron beam and the crystal potential. The real space and reciprocal space information about a crystal structure can be related through the Fourier transform relationships shown below, where () is in real space and corresponds to the crystal potential, and () is its Fourier transform in reciprocal space.
Gold is used for its high electron density which increases electron scatter to give high contrast 'dark spots'. [3] First used in 1971, immunogold labeling has been applied to both transmission electron microscopy and scanning electron microscopy, as well as brightfield microscopy. The labeling technique can be adapted to distinguish multiple ...
The E-T secondary electron detector can be used in the SEM's back-scattered electron mode by either turning off the Faraday cage or by applying a negative voltage to the Faraday cage. However, better back-scattered electron images come from dedicated BSE detectors rather than from using the E–T detector as a BSE detector.