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LB medium bottle and LB agar plate Plate medium agar LB. Lysogeny broth (LB) is a nutritionally rich medium primarily used for the growth of bacteria. Its creator, Giuseppe Bertani, intended LB to stand for lysogeny broth, [1] but LB has also come to colloquially mean Luria broth, Lennox broth, life broth or Luria–Bertani medium. [2]
Super Optimal Broth (SOB medium) is a nutrient-rich bacterial growth medium used for microbiological culture, generally of Escherichia coli. This nutrient-rich microbial broth contains peptides , amino acids , water soluble vitamins and glucose in a low-salt formulation.
The medium must be incubated for a significant length of time, usually four weeks, due to the slow doubling time of M. tuberculosis (15–20 hours) compared with other bacteria. The medium is named after the Austrian pathologist Ernst Löwenstein (1878–1950) and the Danish medical doctor Kai Adolf Jensen (16.7.1894-2.5.1971).
Middlebrook 7H9 broth is a liquid growth medium specially used for culture of Mycobacterium species, notably Mycobacterium tuberculosis. [1] Composition
The medium may be supplemented with blood to facilitate the growth of more fastidious bacteria or antimicrobial agents to permit the selection of various microbial groups from pure microbiota. As with any media, minor changes may be made to suit specific circumstances. TSA is frequently the base medium of other agar plate types.
One hundred grams of raw carrots (about two medium-sized carrots) contains: 48 calories. 0.9 grams of protein. 3.1 grams of fiber. They’re a particularly great source of vitamin A as well.
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The complete medium, with OADC enrichment and PANTA antibiotic mixture, is one of the most commonly used liquid media for the cultivation of mycobacteria. All types of clinical specimens, pulmonary as well as extra-pulmonary (except blood and urine ), can be processed for primary isolation in the MGIT tube using conventional methods.