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The SI unit of molar absorption coefficient is the square metre per mole (m 2 /mol), but in practice, quantities are usually expressed in terms of M −1 ⋅cm −1 or L⋅mol −1 ⋅cm −1 (the latter two units are both equal to 0.1 m 2 /mol).
Variable pathlength absorption spectroscopy uses a determined slope to calculate concentration. As stated above this is a product of the molar absorptivity and the concentration. Since the actual absorbance value is taken at many data points at equal intervals, background subtraction is generally unnecessary.
absorption coefficient is essentially (but not quite always) synonymous with attenuation coefficient; see attenuation coefficient for details; molar absorption coefficient or molar extinction coefficient , also called molar absorptivity , is the attenuation coefficient divided by molarity (and usually multiplied by ln(10), i.e., decadic); see ...
Using this relationship, the set of parameters, the stability constant values and values of properties such as molar absorptivity or specified chemical shifts, may be refined by a non-linear least-squares refinement process. For a more detailed exposition of the theory see Determination of equilibrium constants.
Extinction coefficient refers to several different measures of the absorption of light in a medium: Attenuation coefficient, sometimes called "extinction coefficient" in meteorology or climatology Mass extinction coefficient, how strongly a substance absorbs light at a given wavelength, per mass density
This process is used in analytical chemistry to determine the composition of a mixture of the components of known molar absorptivity spectra. For example, if the heights of two lines are found to be h 1 and h 2, c 1 = h 1 / ε 1 and c 2 = h 2 / ε 2. [14] Parameters of the line shape are unknown.
When an isosbestic plot is constructed by the superposition of the absorption spectra of two species (whether by using molar absorptivity for the representation, or by using absorbance and keeping the same molar concentration for both species), the isosbestic point corresponds to a wavelength at which these spectra cross each other.
This reaction is rapid and stoichiometric, with the addition of one mole of thiol releasing one mole of TNB. The TNB 2− is quantified in a spectrophotometer by measuring the absorbance of visible light at 412 nm, using an extinction coefficient of 14,150 M −1 cm −1 for dilute buffer solutions, [4] [5] and a coefficient of 13,700 M −1 cm −1 for high salt concentrations, such as 6 M ...