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The nucleophilic lysine residue is commonly targeted site in protein bioconjugation, typically through amine-reactive N-hydroxysuccinimidyl (NHS) esters. [3] To obtain optimal number of deprotonated lysine residues, the pH of the aqueous solution must be below the pKa of the lysine ammonium group, which is around 10.5, so the typical pH of the reaction is about 8 and 9.
Although ICEs exhibit various mechanisms promoting their integration, transfer and regulation, they share many common characteristics. ICEs comprise all mobile genetic elements with self-replication, integration, and conjugation abilities, including conjugative transposons, regardless of the particular conjugation and integration mechanism by which they act.
and : are continuous functions on topological spaces, and . being topologically semiconjugate to means, by definition, that is a surjection such that =.. and being topologically conjugate means, by definition, that they are topologically semiconjugate and is furthermore injective, then bijective, and its inverse is continuous too; i.e. is a homeomorphism; further, is termed a topological ...
However, unlike E. coli Hfr conjugation, mycobacterial conjugation is chromosome rather than plasmid based. [7] [8] Furthermore, in contrast to E. coli Hfr conjugation, in M. smegmatis all regions of the chromosome are transferred with comparable efficiencies. The lengths of the donor segments vary widely, but have an average length of 44.2kb.
The conjugate transpose of a matrix with real entries reduces to the transpose of , as the conjugate of a real number is the number itself. The conjugate transpose can be motivated by noting that complex numbers can be usefully represented by 2 × 2 {\displaystyle 2\times 2} real matrices, obeying matrix addition and multiplication: a + i b ≡ ...
The final result of conjugation, transduction, and/or transformation is the production of genetic recombinants, individuals that carry not only the genes they inherited from their parent cells but also the genes introduced to their genomes by conjugation, transduction, and/or transformation. [5] [6] [7]
The Staudinger ligation is a reaction developed by the Bertozzi group in 2000 that is based on the classic Staudinger reaction of azides with triarylphosphines. [15] It launched the field of bioorthogonal chemistry as the first reaction with completely abiotic functional groups although it is no longer as widely used.
Notably, the dose required for the same therapeutic effect was 20 times lower for the developed conjugate (vs. naked mAb). [12] Another reported conjugate, exploiting the same unselective conjugation chemistry, employs an CD44 respectively EphA2 targeting antibody which covalently carries a therapeutically irrelevant “sense-carrier ...